The anti-osteoporosis and antioxidant activities of chemical constituents from Chrysanthemum indicum flowers

Phytother Res. 2015 Apr;29(4):540-8. doi: 10.1002/ptr.5281. Epub 2015 Jan 19.

Abstract

Two new compounds, chrysinoneside A (1) and (-)-trans-chrysanthenol-6-O-β-D-glucopyranoside (2), along with 17 known compounds (3-19) were isolated from Chrysanthemum indicum flowers. The total phenolic and flavonoid contents of various fractions were determined. The EtOAC fraction had the highest total phenolic content (525.84 ± 23.51 mg GAE/g DR) and the total flavonoid content (63.49 ± 3.32 mg QE/g DR). The EtOAc and water fractions showed the greatest peroxyl radical-scavenging capacity and the ability to reduce Cu(I) ions, with ORAC and CUPRAC values ranging from 24.00 ± 0.44 to 28.06 ± 1.35 and 16.90 ± 0.51 to 49.77 ± 0.97 μM, respectively. Compounds 5-11, 18, and 19 displayed strong effects in both peroxyl radical-scavenging and reducing capacity assays at a concentration of 10 μM. The anti-osteoporosis activity of these compounds was also evaluated. Compounds 10, 13, and 19 exhibited the most potent tartrate-resistant acid phosphatase activity in receptor activator of nuclear factor-κB ligand-induced osteoclastic RAW 264.7 cells with values of 105.95 ± 1.18, 110.32 ± 3.95, and 112.58 ± 6.42%, respectively.

Keywords: (−)-trans-Chrysanthenol-6-O-β-D-glucopyranoside; Chrysanthemum indicum; anti-osteoporosis activity; antioxidant activity; chrysinoneside A.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acid Phosphatase / metabolism
  • Animals
  • Antioxidants / pharmacology*
  • Cell Differentiation / drug effects
  • Cell Line
  • Chrysanthemum / chemistry*
  • Flavonoids / pharmacology*
  • Flowers / chemistry
  • Isoenzymes / metabolism
  • Mice
  • Molecular Structure
  • Osteoclasts / drug effects*
  • Osteoclasts / metabolism
  • Osteoporosis
  • Oxidation-Reduction
  • Phenols / pharmacology*
  • Plant Extracts / pharmacology*
  • RANK Ligand / metabolism
  • Tartrate-Resistant Acid Phosphatase

Substances

  • Antioxidants
  • Flavonoids
  • Isoenzymes
  • Phenols
  • Plant Extracts
  • RANK Ligand
  • Tnfsf11 protein, mouse
  • Acid Phosphatase
  • Tartrate-Resistant Acid Phosphatase